A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E
نویسندگان
چکیده
منابع مشابه
Infectivity of human coronavirus strain 229E.
The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h after infection in cells infected with high dilutions of human coronavirus 229...
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The genomic RNA of human coronavirus strain 229E (HCV 229E) migrated on polyacrylamide gels as a single peak with a mol. wt. of 5.8 X 10(6). Denaturation of the genome with formaldehyde did not alter its electrophoretic mobility, which suggests that the HCV 229E genome is a single-stranded molecule. At least 30% of the genomic RNA was shown to contain covalently attached polyadenylic acid [poly...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2020
ISSN: 1743-422X
DOI: 10.1186/s12985-020-01405-y